Problems with Pyautogui and GUi

I have tries for past year to recreate your basic code and using techmoto code to help me understand it. I have consulted with chatgpt but still not getting the results I wish for. I can not seem to get the gui to continuously work...it freezes once the frames run and pyautogui will not respond in the main program even though I'm importing it into the main via a liberary.

Any help would be appreciated.

Thanks